A Spanish health worker, who works at a Madrid hospital where two ebola patients died, tests positive for the deadly disease.
A Spanish nurse has become the first person to contract ebola outside of West Africa during an outbreak that has killed more than 3,400 people.
The woman was part of a medical team at Madrid's La Paz-Carlos III hospital that treated two missionaries, who died shortly after being repatriated from Africa with the disease.
Health Minister Ana Mato said an emergency protocol had been put in place and authorities were working to establish the source of the contagion.
"We are working to guarantee the safety of all citizens," she said.
Spanish priest Miguel Pajares, 75, was infected with ebola in Liberia and died at the hospital on 12 August.
Another Spanish missionary, Manuel Garcia Viejo, 69, was repatriated from Sierra Leone and died at the hospital on 25 September.
Both were members of a Roman Catholic group that runs a charity working with ebola victims in Africa.
A Spanish health official said 30 medical staff who treated the two priests are being monitored.
The infected nurse began to feel ill on 30 September, but did not go to hospital until Sunday complaining of a fever.
The assistant nurse, who is married without children, is being treated in isolation at a hospital in a southern Madrid suburb.
Health authorities are trying to track down all the people she may have come in contact with since she contracted the disease.